biological source
human blood
packaging
tube of 5 μg 94111519-DNA-5UG, pkg of vial of cells 94111519-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Immunoglobulin G (IgG1) (kappa) specific for Hepatitis C virus NS4 region
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Used in HCV typing studies.
Biochem/physiol Actions
Derived from a patient with chronic Hepatitis C (HCV type 1). Antibody specific to non structural protein 4a (NS4a) of Hepatitis C virus. Fine specificity mapped to amino acid residues 1697-1708 of HCV deduced amino acid sequence. The cells should be handled under laboratory containment level 2 conditions.
Human anti-hepatitis C virus IgG1 secreting B cell
Preparation Note
Maintain cultures between 3-9x10,000 cells/ml; using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 20u/ml IL-6 + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
法规信息
常规特殊物品
低风险生物材料
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