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UNSPSC Code:
41106514
Biological source:
Horse x Mouse/Horse Hybridoma
Technique(s):
cell culture | mammalian: suitable
Myeloma:
AFRC 17D1
Reactivity:
Anti-Haemagglutinin, influenza
biological source
Horse x Mouse/Horse Hybridoma
packaging
tube of 5 μg 94120826-DNA-5UG, pkg of vial of cells 94120826-1VL
ImmunoDon
Horse peripheral lymph node
Myeloma
AFRC 17D1
Reactivity
Anti-Haemagglutinin, influenza
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Immunogen
Influenza A equine 2 virus
Application
Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.
Biochem/physiol Actions
Horse peripheral lymph node x AFRC 17D1
The heterohybridoma AFRC 56D2 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 56D2 horse cells from the peripheral lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Other Notes
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法规信息
低风险生物材料
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