COR-L23/R

Human Caucasian lung large cell carcinoma

The cell line COR-L23/R is a multi-drug resistant (MDR) sub-line derived from the parent line COR-L23 (Sigma Catalogue number. 92031919) after treatment with doxorubicin (adriamycin). The P-glycoprotein negative cell line is cross resistant to daunorubicin, vincristine and rhodamine 123. Drug resistance is caused by enhanced drug efflux that can be inhibited by addition of buthionine sulphoximide (BSO), an inhibitor of glutathione synthesis. COR-L23/R contain reduced levels of glutathione and glutathione-S-transferase activity compared to the parent line. MDR seems to be connected with overexpression of multi-drug resistance-associated protein (MRP) which is discussed as a drug transporter.

Study of drug resistance, analysis of sub-cellular localisation of drugs, drug transport

RPMI 1640 + 2mM Glutamine + 0.2μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). Start without drug. Add drug after first passage and then add at all subsequent passages.

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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