生物来源
human skin
描述
Human malignant melanoma IL-1 insensitive
包装
tube of 5 μg 97011320-DNA-5UG
pkg of vial of cells 97011320-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human malignant melanoma IL-1 insensitive
细胞系描述
The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.
应用
IL-1 resistance studies, receptor studies
培养基
RPMI 1640 + 2mM Glutamine + 10% FBS
传代培养常规
Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
其他说明
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