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关于此项目
UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG2a
Technique(s):
cell culture | mammalian: suitable
Myeloma:
Sp2/0-Ag14
Reactivity:
Anti-HIV-1 gp120
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 97040308-DNA-5UG, pkg of vial of cells 97040308-1VL
ImmunoDon
Mouse BALB/c spleen
Myeloma
Sp2/0-Ag14
Reactivity
Anti-HIV-1 gp120
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
rgp 120
Application
gp120 assays
Biochem/physiol Actions
Mouse x Mouse hybridoma
The hybridoma cell line 55-6 produces antibodies reacting with the Human Immunodeficiency Virus I (HIV-1) envelope protein, gp120. Spleen cells from BALB/c mice that had been sequentially immunised with recombinant gp120(rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB,SF2 and Z6) have been fused with Sp2/0-Ag14 myeloma cells. The antibody binds rgp120 from the III B isolate and reacts weakly with gp120 from the MN strain, both shown by ELISA. No cross blocking of any other antibodies in the developed panel was detected, thus indicating that 55-6 binds to a unique site on gp120IIIB. In peptide recognition assays it was shown to bind a synthetic peptide 105-117 (in the CI region) of gp120.
Preparation Note
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2;37ºC
RPMI 1640 + 2mM glutamine + HT + 10% FBS
Other Notes
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法规信息
低风险生物材料
此项目有
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