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UNSPSC Code:
41106514
Biological source:
Mouse x Mouse Hybridoma
Isotype:
IgG2a
Technique(s):
cell culture | mammalian: suitable
Myeloma:
NS-1
Reactivity:
Anti-WC1, bovine/porcine
biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 99011338-DNA-5UG, pkg of vial of cells 99011338-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
NS-1
Reactivity
Anti-WC1, bovine/porcine
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Immunogen
bovine Con-A- stimulated lymphoblasts
Application
Flow cytometry, Immunohistology, Immunoprecipitation
Biochem/physiol Actions
Mouse x Mouse hybridoma
The monoclonal antibody CC101 was produced by inoculating mice with bovine lymphocytes activated with concanavalin A (Con A) The antibody is specific for the WC1 molecule on gamma delta T lymphocytes in cattle that also reacts with porcine, sheep and goat cells. It recognizes a 180,000 MW molecule on the majority of pig CD2- CD4- CD8- T cells. Cells have been grown on macrophage feeder cells and using hypoxanthine and thymidine at concentrations appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
Preparation Note
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 10% FBS and HT supplement
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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