生物来源
Mouse x Mouse Hybridoma
包装
tube of 5 μg 99011338-DNA-5UG
pkg of vial of cells 99011338-1VL
ImmunoDon
BALB/c mouse spleen
骨髓瘤
NS-1
反应性
Anti-WC1, bovine/porcine
技术
cell culture | mammalian: suitable
同位素/亚型
IgG2a
运输
dry ice
储存温度
−196°C
免疫原
bovine Con-A- stimulated lymphoblasts
应用
Flow cytometry, Immunohistology, Immunoprecipitation
生化/生理作用
Mouse x Mouse hybridoma
The monoclonal antibody CC101 was produced by inoculating mice with bovine lymphocytes activated with concanavalin A (Con A) The antibody is specific for the WC1 molecule on gamma delta T lymphocytes in cattle that also reacts with porcine, sheep and goat cells. It recognizes a 180,000 MW molecule on the majority of pig CD2- CD4- CD8- T cells. Cells have been grown on macrophage feeder cells and using hypoxanthine and thymidine at concentrations appropriate for hybridomas. It is recommended to continue this until sufficient cells have been frozen.
制备说明
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 10% FBS and HT supplement
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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