产品名称
STEADY电感受态细胞, for plasmid production using unstable DNA
biological source
Escherichia coli
grade
Molecular Biology
form
buffered aqueous solution
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Features and Benefits
- Clone unstable sequences with reliable results
- Available as chemically competent or electrocompetent cells
- Your choice of two packaging formats
- High efficiency: over 1 × 108 cfu/μg (chem) or 1 × 1010 cfu/μg (electro)
- Excellent value
General description
The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.
Genotype
recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.
Genotype
recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)
Other Notes
- STEADY electrocompetent cells
- pUC 19 transformation control DNA
- recovery medium for cloning
存储类别
10 - Combustible liquids
法规信息
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