用途
sufficient for 100 electro-transformations (40 μl)
储存温度
2-8°C
应用
All the reagents needed for fast, cost-effective production of frozen bacterial cell stocks for transformations.
生化/生理作用
Typical transformation efficiency is ≥108 transformants per μg using E. coli DH5α and pUC19 plasmid DNA. The frozen stocks can be used immediately or stored at −70°C.
法规信息
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W J Dower et al.
Nucleic acids research, 16(13), 6127-6145 (1988-07-11)
E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5
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