用途
sufficient for 100 tests
储存温度
2-8°C
应用
FLICA Caspase Assay detects active caspases inside the cell using novel Fluorochrome Inhibitor of Caspases (FLICA)-cell permeable and non-cytotoxic caspase inhibitors. Once inside the cell, the FLICA probe covalently binds to a reactive cysteine residue on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. Bound FLICA is retained within the cell, while any unbound reagent is washed away. The bound FLICA emits a green fluorescent signal, which is a direct measurement of the number of active caspase molecules that are present in the cell at the time the reagent was added.
Fluorometric detection of active cellular caspase 1 using cell-permeable, non-toxic fluorochrome inhibitor of caspase 1 FAM-YVAD-FMK, which is carboxyfluorescein (FAM) derivative of valylalanylaspartic acid (VAD) fluoromethyl ketone (FMK) that preferentially binds covalently to the active caspase 1 and is visualized by fluorometry, fluorescence microscopy, or flow cytometry.
特点和优势
The FLICA Assays offer:
- Potent, cell-permeable and non-toxic fluorochrome inhibitor
- Preferential detection of selected caspases based on inhibitor specificity
- A direct measure of apoptosis expressed as the number of active caspase enzymes present in the cell
免责声明
Once reconstituted, the unused 150X FLICA stock should be stored in aliquots at −20 °C protected from light.
仅试剂盒组分
产品编号
说明
- FAM-YVAD-FMK FLICA Reagent 4 vial(s)
- Wash Buffer, Concentrate 10X 60 mL
- Fixative 6 mL
- Propidium iodide 1 mL
- Hoechst Stain 33342 1 mL
法规信息
新产品
此项目有
William G Harter et al.
Bioorganic & medicinal chemistry letters, 14(3), 809-812 (2004-01-27)
A series of sulfonamides (1) has been prepared as inhibitors of interleukin-1beta converting enzyme (ICE), also known as caspase 1. These compounds were designed to improve potency by rigidifying the enzyme bound molecule through an intramolecular hydrogen bond. An X-ray
Catharina Lindberg et al.
Journal of neuroimmunology, 146(1-2), 99-113 (2003-12-31)
Caspase-1/interleukin-1beta (IL-1beta)-converting enzyme (ICE) cleaves IL-1beta and IL-18 precursor proteins to the active forms of these proinflammatory cytokines. Since both cytokines are constitutively expressed in the brain, we investigated whether this is also the case for caspase-1. Using an antibody
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