Anti-Human CD10 (MEM-78) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Neprilysin (UniProt: P08473; also known as EC:3.4.24.11, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 24.11, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10) is encoded by the MME (also known as EPN) gene (Gene ID: 4311) in human. CD10 is a single-pass type II membrane glycoprotein of the peptidase M13 family that has a cytoplasmic domain (aa 2-28), a transmembrane domain (aa 29-51), and a long extracellular domain (aa 52-750). It is highly expressed on epithelial cells of lung, intestine, kidney, breast, placenta. CD10 displays thermolysin-like specificity, but is almost confined to acting on polypeptides of up to 30 amino acids. It plays an important role in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. It is also reported to degrade atrial natriuretic factor as well as brain natriuretic factor. Neprilysin is also reported to protect neurons against amyloid peptide toxicity. It is considered as a cell surface marker in the diagnosis of acute lymphocytic leukemia. Glycosylation of CD10 at Asn-628 is shown to be essential for its surface expression and neutral endopeptidase activity. Mutations in MME gene have been linked to Charcot-Marie-Tooth disease that is characterized by progressive weakness and atrophy of peroneal muscles and distal muscles of the arms. (Ref.: Higuchi, Y., et al. (2016). Ann. Neurol. 79(4); 659-672; Wisner, A., et al. (2006). Proc. Natl. Acad. Sci. USA. 103(47); 17979-17984).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

NALM-6 human pre-B cells.

Quality Control Testing:

Evaluated by Flow Cytometry in NALM-6 cell line.

Flow Cytometry Analysis: Staining of one million human NALM-6 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1045, Anti-Human CD10 (MEM-78) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone MEM-78 is a mouse monoclonal antibody that detects human Naprilysin (CD10). It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.