Anti-Human HLA-DRA (LN3) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

HLA class II histocompatibility antigen, DR alpha chain (UniProt: P01903; also known as MHC class II antigen DRA) is encoded by the HLA-DRA (also known as HLA-DRA1) gene (Gene ID: 3122) in human. HLA-DRA is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 26-216), a transmembrane domain (aa 217-239), and a short cytoplasmic tail (aa 240-254). It is expressed in macrophages, dendritic cells, B cells, and thymic epithelial cells. In complex with the b-chain (HLA-DRB), it displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by a- b T cell receptor (TCR) on HLA-DR-restricted CD4+ T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically, it presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. It also presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Activated Human peripheral lymphocytes.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood lymphocytes.

Flow Cytometry Analysis: Staining of one million Human peripheral blood lymphocytes (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1090, Anti-Human HLA-DRA (LN3) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone LN3 is a mouse monoclonal antibody that detects HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.