Anti-Mouse CD11a (M17/4) ColorWheel^® Dye-Ready mAb

ColorWheelColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheelColorWheel^® antibody with any ColorWheelColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheelColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheelColorWheel^® technology requires both ColorWheelColorWheel^® antibody and ColorWheelColorWheel^® dye for Flow Cytometry application.

Integrin alpha-L (UniProt: P24063; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, Lymphocyte antigen 15, Ly-15, CD11a) is encoded by the Itgal (also known as Lfa-1, Ly-15) gene (Gene ID: 16408) in murine species. Integrins are part of a family of heterodimeric transmembrane glycoproteins that recognize a variety of ligands and control numerous physiologic functions in cells. CD11a is a single-pass type I membrane glycoprotein protein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form that contains a large extracellular domain (aa 24-1087), a transmembrane domain (aa 1088-1108), and a cytoplasmic domain 1109-1163). CD11a noncovalently associates with b2 integrin to forms a heterodimer to serve as a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils, and eosinophils. It is also involved in generation of common lymphoid progenitor cells in bone marrow. In resting T-cells, about 40% of CD11a is constitutively phosphorylated and phosphorylation causes conformational changes needed for ligand binding and is considered to be essential for activation by some physiological agents. CD11a -/- mice exhibit decreased thymic cellularity and impaired early T cell development. CD11a is shown to be critical for generation of common lymphoid progenitors (CLPs) and lymphoid-primed multipotent progenitors (LMPPs). (Ref.: Bose, TO., et al. (2014). J. Immunol. 193(6); 2863-2872).

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C57BL/6 Mouse Splenic Secondary Cytotoxic T Lymphocytes.

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis:

Staining of one million C57BL/6 mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1121, Anti-Mouse CD11a (M17/4) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1018 Rat IgG2a isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1121, Anti-Mouse CD11a (M17/4) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1018 Rat IgG2a isotype control and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone M17/4 is a rat monoclonal antibody that detects murine CD11a (Integrin aL). It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.