Anti-Mouse FcεR1 (MAR-1) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

High affinity immunoglobulin epsilon receptor subunit alpha (UniProt: P20489; also known as Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha) is encoded by the Fcer1a (also known as Fce1a) gene (Gene ID:14125) in murine species. Fc R1 is a tetrameric immune recognition receptor that is composed on one -chain, one β-chain and two disulfide-linked -chains ( β 2). The -chain contains the binding site for IgE but lacks intrinsic signaling ability that is carried out by the β- and -subunits. Fc RIa is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature -chain that contains an extracellular domain (aa 23-204), a transmembrane domain (a 205-223), and a cytoplasmic domain (aa 224-250). It also contains two Ig-like domains (aa 28-104 and 114-181) in its extracellular domain. It is expressed in bone marrow mast cells and basophils and its level is upregulated by the presence of IgE. In pineal gland its expression is increased at night. It serves as a high affinity receptor and binds to the Fc region of immunoglobulins. It is responsible for starting the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators such as histamine that is responsible for the manifestations of allergy symptoms. (Ref.: Daeron, M. (1997). Annu. Rev. Immunol. 15; 203-243; Dombrowicz, D., et al. (1993). Cell. 75(5); 969-976).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Mouse Fc R1a. The exact immunogen information is proprietary.

Quality Control Testing

Evaluated by Flow Cytometry in MC/9 cells.

Flow Cytometry Analysis: Staining of one million MC/9 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1137, Anti-Mouse Fc R1 (MAR-1) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million MC/9 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1137, Anti-Mouse Fc R1 (MAR-1) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Clone MAR-1 is an Armenian Hamster monoclonal antibody that detects Mouse High affinity immunoglobulin epsilon receptor subunit alpha (FC R1a).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.