Anti-Mouse CD49b (HMa2) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Integrin alpha-2 (UniProt: Q62469; also known as CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, GPIa, VLA-2 subunit alpha, CD49b) is encoded by the Itga2 gene (Gene ID: 16398) in murine species. Integrins are heterodimers composed of a variable -subunit and a conserved β- subunit. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various - and β-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the -subunit. Integrins bind to their ligands with a low affinity and this binding occurs only when a certain minimal number of integrins are present at specific points known as focal contacts and hemidesmosomes. In response to specific stimuli, they cluster in focal contacts and their combined affinities create a region on the cell surface, which presents sufficient adhesive capacity to adhere to the ECM. This allows cells to bind to a large numbers of matrix molecules simultaneously while still maintaining their ability to explore their environment without losing all attachments. CD49b is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to produce the mature protein that contains a large extracellular domain (aa 27-1129), a transmembrane domain (aa 1130-1151), and a short cytoplasmic domain (aa 1152-1178). CD49b/CD29 (integrin β1) complex serves as a collagen receptor, being responsible for adhesion of platelets and other cells to collagen, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It also serves as a receptor for laminins. This complex is expressed by some CD4-positive T cells, natural killer cells, epithelial cells, and platelets.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Mouse colon carcinoma cell line Colon26

Quality Control Testing

Evaluated by Flow Cytometry in C57Bl/6 mouse splenocytes.

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse splenocytes was performed with APC-conjugated anti-mouse CD161/NK1.1 and with 5 μL of a 1:1 mixture of Cat. No. CWA-1140, Anti-Mouse CD49b (HMa2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD49b expression was observed in CD161/NK1.1 positive population.

Tested Applications

Flow Cytometry Analysis: Staining of one million C57BL/6 mouse splenocytes was performed with PE-conjugated anti-mouse CD161/NK1.1 and with 5 μL of a 1:1 mixture of Cat. No. CWA-1140, Anti-Mouse CD49b (HMa2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD49b expression was observed in CD161/NK1.1 positive population.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.