Anti-Human MCAM/CD146 (P1H12) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Cell surface glycoprotein MUC18 (UniProt: P43121; also known as Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146) is encoded by the MCAM (also known as MUC18) gene (Gene ID: 4162) in human. MCAM/CD146 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 24-559), a transmembrane domain (aa 560-583), and a cytoplasmic domain (aa 584-646). It contains two Ig-like V-type domains in its N-terminal half and three Ig-like C2-type domains. It is detected in endothelial cells in vascular tissue throughout the body and plays a key role in endothelial signaling by interacting with a variety of ligands. It is involved in angiogenesis, vessel permeability, and leukocyte transmigration. During embryonic migration it is also observed at the surface of neural crest cells. It plays a role in cell adhesion and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. It acts as a surface receptor that triggers tyrosine phosphorylation of Fyn and FAK1 and causes a transient increase in intracellular calcium levels. MCAM/CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma. It is strongly expressed on metastatic lesions and advanced primary tumors. Three forms of CD146 have been described: 2 membrane isoforms, CD146 long (lgCD146) and CD146 short (shCD146), which differ in the cytoplasmic portion, and a soluble form (sCD146). (Ref.: Leroyer AS., et al. (2019). Arterioscler. Thromb. Vasc. Biol. 39(6);1026-1033; Lehmann, JM., et al. (1989). Proc. Natl. Acad. Sci. USA. 86(24); 9891-9895).

This ColorWheel^® Dye-Ready monoclonal antibody was developed using our MAB16985, Anti-MCAM Antibody, clone P1H12, mouse monoclonal antibody.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Cultured Human umbilical vein endothelial cells (HUVEC).

Quality Control Testing

Evaluated by Flow Cytometry in Human umbilical vein endothelial cells (HUVEC).

Flow Cytometry Analysis: Staining of one million Human umbilical vein endothelial cells (HUVEC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1142, Anti-Human MCAM (P1H12) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human umbilical vein endothelial cells (HUVEC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1142, Anti-Human MCAM (P1H12) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-101 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone P1H12 is a mouse monoclonal antibody that detects Cell surface glycoprotein MUC18 (MCAM/CD146).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.