Anti-Human HER-2/CD340 (24D2) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Receptor tyrosine-protein kinase erbB-2 (UniProt: P04626; also known as EC:2.7.10.1, Metastatic lymph node gene 19 protein, MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, CD340) is encoded by the ERBB2 (also known as HER2, MLN19, NEU, NGL) gene (Gene ID: 2064) in human. ErbB2 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 23-652), a transmembrane domain (aa 653-675), and a cytoplasmic domain (aa 676-1255). Its tyrosine kinase domain is localized to amino acids 720-987. It can also be translocated to the nucleus and its nuclear localization signal resides in amino acids 676-689. It has intrinsic protein tyrosine kinase activity, and it mediates the lateral signal transduction of all ErbB receptors. It is involved in in the regulation of a variety of cellular functions, including cell growth, differentiation, and apoptosis. It also regulates outgrowth and stabilization of peripheral microtubules. In the nucleus it is involved in transcriptional regulation and associates with the 5′-TCAAATTC-3′ sequence in the PTGS2/COX-2 promoter and activates its transcription. It is also implicated in transcriptional activation of p21. ERBB2 gene is reported to be overexpressed in about 30% of all human breast carcinomas. ErbB2 is activated by dimerization and autophosphorylation. Ligand-binding is shown to increase phosphorylation on tyrosine residues. (Ref.: Joshi, SK., et al. (2020). Leukemia. 34(10); 2798-2804).

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NIH3T3 cells transfected with Human HER-2 (CD340).

Quality Control Testing

Evaluated by Flow Cytometry in MCF-7 cells.

Flow Cytometry Analysis: Staining of one million MCF-7 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1149, Anti-Human HER-2/CD340 (24D2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million MCF-7 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1147, Anti-Human CD147 (MEM-M6/1) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of the equivalent amount of Cat. No. CWA-101 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone 24D2 is a mouse monoclonal antibody that detects human HER-2 (CD340). It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.