Anti-Human CDK1 (POH-1) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Cyclin-dependent kinase 1 (UniProt: P06493; also known as EC:2.7.11.22, EC:2.7.11.23), CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase) is encoded by the CDK1 ( also known as CDC2, CDC28A, CDKN1, P34CDC2) gene (Gene ID: 983) in human. CDK1 is an archetypical kinase that forms a stable, but non-covalent complex with a regulatory subunit and with a cyclin. It interacts with cyclins-B to form a serine/threonine kinase holoenzyme complex known as the maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. It can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Via these interactions, it plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset. It promotes G2-M transition and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. CDK1 is mostly cytoplasmic during the interphase and is reversibly translocated from cytoplasm to nucleus when phosphorylated before the G2-M transition. It can undergo phosphorylation at multiple sites and each phosphorylation has a specific effect. Phosphorylation at Thr 161 by CAK/CDK7 is reported to activate its kinase activity and phosphorylation at Thr 14 and Tyr 15 by PYMYT1, a member of the WEE kinase family, prevents its nuclear translocation and inactivates this enzyme. Phosphorylation at Tyr 15 by WEE1 and WEE2 inhibits its protein kinase activity and blocks its entry into mitosis. This keeps CDK1-Cyclin B complex in an inactive state until the end of G2 phase. By the end of G2 phase, PKMYT1 and WEE1 are inactivated and CDC25A and CDC25B are activated, which dephosphorylate Thr 14 and Tyr 15 leading to CDK1 activation. (Ref.: Diril, MK., et al., (2012). Proc. Natl. Acad. Sci. USA. 109(10); 3826-3831; Draetta, G., and Beach, D. (1988). Cell. 54(1); 17-26).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Full-length recombinant human CDK1.

Quality Control Testing

Evaluated by Flow Cytometry in fixed and permeabilized HeLa cells.

Flow Cytometry Analysis: Staining of one million fixed and permeabilized HeLa cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1156, Anti-Human CDK1 (POH-1) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1016 Mouse IgG2a isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million fixed and permeabilized HeLa cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1156, Anti-Human CDK1 (POH-1) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1016 Mouse IgG2a isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone POH-1 is a mouse monoclonal antibody that detects Cyclin-dependent kinase 1 (CDK1).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.