Anti-Human β2-Microglobulin (B2M-01) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Beta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. β-2-microglobulin is a ubiquitously expressed component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is produced at a constant rate and freely filtered by the kidney. It is completely reabsorbed by megalin-mediated endocytosis on the proximal tubules and then completely catabolized within the tubular cell. Only a very tiny amount (0.03%) of β-2-microglobulin is excreted in the urine. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). β-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum β-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin′s lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency. (Ref.: Sabin, CA., et al. (1994). Br. J. Haematol. 86(2); 366-371; Karlsson, FA., et al. (1980). Scand. J. Clin. Lab. Invest. Suppl 154: 27-37).

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Full-length purified human β2-Microglobulin.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC), gated on lymphocytes.

Flow Cytometry Analysis: Staining of one million human peripheral blood mononuclear cells (PBMC) lymphocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1161, Anti-Human ?2-Microglobulin (B2M-01) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million human peripheral blood mononuclear cells (PBMC) lymphocytes was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1161, Anti-Human ?2-Microglobulin (B2M-01) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone B2M-01 is a mouse monoclonal antibody that detects β-2-microglobulin.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.