Anti-Human CD94/KLRD1 (HP-3D9) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Natural killer cells antigen CD94 (UniProt: Q13241; also known as KP43, Killer cell lectin-like receptor subfamily D member 1, NK cell receptor, CD94) is encoded by the CD94 (also known as KLRD1) gene (Gene ID: 3824) in human. CD94 is a single-pass type II membrane protein that contains a cytoplasmic domain (aa 1-10), a transmembrane domain (aa 11-31), and an extracellular domain (aa 32-179). It also contains a C-type lectin domain (aa 68-175). CD94 expression is observed in subsets of NK cells, memory/effector CD8+ , β T cells, and melanoma-specific cytotoxic T cells. It is also expressed in terminally differentiated cytotoxic , T cells. It serves as an immune inhibitory receptor that is involved in self-non-self-discrimination. It serves primarily as a ligand binding subunit as it lacks the capacity to signal. In complex with KLRC1 (Cd159a) or KLRC2 (CD159c) on cytotoxic and regulatory lymphocyte subsets, it recognizes non-classical major histocompatibility (MHC) class Ib molecule HLA-E that is loaded with self-peptides derived from the signal sequence of classical MHC class Ia and non-classical MHC class Ib molecules. It enables cytotoxic cells to monitor the expression of MHC class I molecules in healthy cells and to tolerate self. CD94 is also reported to dominantly counteract T cell receptor signaling on a subset of memory/effector CD8+ T cells as part of an antigen-driven response to avoid autoimmunity. (Ref.: Bhagat, G., et al. (2008). J. Clin. Invest. 118(1); 281-293; Braud, VM., et al. (1998). Nature. 391(6669); 795-799).

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Cultured human NK cells.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC), gated on lymphocytes.

Flow Cytometry Analysis: Staining of one million human peripheral blood mononuclear cells (PBMC), lymphocytes was performed using FITC conjugated anti-human CD56 and 5 µL of a 1:1 mixture of Cat. No. CWA-1165, Anti-Human CD94/KLRD1 (HP-3D9) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD94 expression was observed mainly in CD56 positive population.

Tested Applications

Flow Cytometry Analysis: Staining of one million human peripheral blood mononuclear cells (PBMC), lymphocytes was performed using FITC conjugated anti-human CD56 and 5 µL of a 1:1 mixture of Cat. No. CWA-1165, Anti-Human CD94/KLRD1 (HP-3D9) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD94 expression was observed mainly in CD56 positive population.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone HP-3D9 is a mouse monoclonal antibody that detects CD94/KLRD1. It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.