Anti-Human CD23 (EBVCS-5) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Low affinity immunoglobulin epsilon Fc receptor (UniProt: P06734;also known as BLAST-2;C-type lectin domain family 4 member J;Fc-epsilon-RII;Immunoglobulin E-binding factor;Lymphocyte IgE receptor;CD23) is encoded by the FCER2 (also known as CD23A;CLEC4J;FCE2;IGEBF) gene (Gene ID: 2208) in human. CD23 is a homotrimeric;single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-21);a transmembrane domain (aa 22-47);and an extracellular domain (aa 48-321). that serves as a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. CD23 can also exist as a soluble excreted form;sCD23 (aa 150-321);which is produced by ADAM10-mediated ectoderm shedding and has pleiotropic cytokine-like activities. CD23 plays an essential role in the regulation of IgE production and in the differentiation of B-cells. Its expression is observed by subsets of B cells;monocytes;follicular dendritic cells;platelets;and eosinophils. It has a large C-terminal globular extracell

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Epstein-Barr Virus transformed cell line.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes.

Flow Cytometry Analysis: Staining of one million human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed with APC conjugated anti-human CD19 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1182, Anti-Human CD23 (EBVCS-5) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD23 expression was observed in CD19 positive population.

Tested Applications

Flow Cytometry Analysis: Staining of one million human PBMC lymphocytes was performed with PE conjugated anti-human CD19 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1182, Anti-Human CD23 (EBVCS-5) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or the equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD23 expression was observed in CD19 positive population.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone EBVCS-5 is a mouse monoclonal antibody that detects CD23. It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.