Anti-Human CD5 (UCHT2) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

T-cell surface glycoprotein CD5 (UniProt: P06127;also known as Lymphocyte antigen T1/Leu-1;CD5) is encoded by the CD5 (also known as LEU1) gene (Gene ID: 921) in human. CD5 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-24);which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 25-372);a transmembrane domain (aa 373-402);and a cytoplasmic domain (aa 403-495). The extracellular region contains three scavenger receptor cysteine rich (SRCR) domains. CD5 is expressed on the surface of all T cells bearing the β heterodimer T cell receptor (TCR) but is absent or present at lower density on the T cell subset. It is involved in the regulation of T cell function and proliferation. It is also detected on a subset of B cells;specifically the B1 cells. However;B cells usually express two-to-five-fold fewer CD5 molecules per cell than T cells. In B cells;CD5 promotes constitutive activation of pERK1/2

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Thymocytes and Sézary T cells.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes.

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed with APC conjugated anti-human CD19 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1183, Anti-Human CD5 (UCHT2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye. CD5 expression was mostly observed in CD19 negative population.

Tested Applications

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) gated on lymphocytes was performed with PE conjugated anti-human CD19 and with 5 µL of a 1:1 mixture of Cat. No. CWA-1183, Anti-Human CD5 (UCHT2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye. CD5 expression was mostly observed in CD19 negative population.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone UCHT2 is a mouse monoclonal antibody that detects CD5. It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.