Anti-Human β-Tubulin (AA2) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

Tubulin beta chain (UniProt: P07437/Q2KJD0;also known as Tubulin beta-5 chain) is encoded by the TUBB gene (Gene ID: 203068/615087) in human and bovine. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed of /β-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. /β heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the β-tubulin subunit facing the microtubule plus end conferring a structural polarity. Tubulin binds two moles of GTP;one at an exchangeable site on the β-chain and one at a non-exchangeable site on the C-chain. Tubulin dimers constantly polymerize and depolymerize;and MTs can undergo rapid cycles of assembly and disassembly. The first stage of MT formation;the nucleation phase;is slow. In the presence of Mg2+ and GTP;- and β- tubulins join in an end-to-end manner to form protofilaments with altern

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Purified β-tubulin from bovine brain.

Quality Control Testing

Evaluated by Flow Cytometry in fixed and permeabilized SH-SY5Y cells.

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized SH-SY5Y cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1184, Anti-Human ?-Tubulin (AA2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized SH-SY5Y cells was performed using 5 µL of a 1:1 mixture of Cat. No. CWA-1184, Anti-Human ?-Tubulin (AA2) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone AA2 is a mouse monoclonal antibody that detects β-tubulin. It targets an epitope within 19 amino acids from the C-terminal region.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 µL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 µL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.