Lambda DNA Pst I Digest

Pst I digested bacteria phage Lambda DNA is suitable for size determination of double-stranded DNA using DNA electrophoresis. Some of the 29 fragments are seen as heavy bands which are actually composed of doublets or triplets. The recommended agarose gel concentration is 1.0% for this marker. A sample of the marker should be diluted with gel loading buffer to an appropriate loading concentration. Typically 500 ng per well (50 ng/μl, 10 μl load) is sufficient to be seen using ethidium bromide staining.

Solution in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA

Contains 29 fragments, 15–11,497 bp