浓度
4 units/reaction (50 μl reaction volume)
运输
wet ice
储存温度
−20°C
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应用
KlenTaq® DV ReadyMix™ is an excellent blend for applications requiring high PCR yields from less complex, thoroughly purified templates.
生化/生理作用
KlenTaq-1 is more efficient and more processive than either native Taq DNA polymerase or other N-terminal deletions of Taq. This means a higher amount of product is generated with the same number of cycles than when using Taq DNA polymerase.
In RED KlenTaq DV ReadyMix, the inert red dye allows for easy confirmation of enzyme addition and visualization of complete mixing during setup. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. There is no need to add a loading buffer/tracking dye prior to electrophoresis. The red dye migrates at approximately the same rate as a 125 base pair fragment.
In RED KlenTaq DV ReadyMix, the inert red dye allows for easy confirmation of enzyme addition and visualization of complete mixing during setup. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. There is no need to add a loading buffer/tracking dye prior to electrophoresis. The red dye migrates at approximately the same rate as a 125 base pair fragment.
特点和优势
- Better efficiency and yield than Taq-based formulations, due to the higher processivity of KlenTaq-1
- Magnesium optimization is unnecessary, since KlenTaq works over a broad magnesium range
- Excellent amplification up to 3 kb for less complex templates (e.g., plasmid and lambda DNA) and 1.5 kb on human genomic DNA
- When performing large numbers of PCR reactions, KlenTaq DV ReadyMixes can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent performance
- RED KlenTaq DV ReadyMix provides immediate confirmation that not only has the enzyme been added, but that proper mixing has occurred. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis
其他说明
KlenTaq® DV ReadyMix™ mixes combine the performance benefits of KlenTaq DNA polymerase with the convenience of a ReadyMix. This ready-to-use mixture of KlenTaq-1 DNA polymerase (a 5′-exo-minus, N-terminal deletion of Taq DNA polymerase), 99% pure deoxynucleotides, reaction buffer and a small amount of a proofreading DNA polymerase is provided in a 2× concentrate. RED KlenTaq DV ReadyMix includes Sigma′s proprietary inert red dye.
For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Reaction volumes can be scaled down, if desired.
For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Reaction volumes can be scaled down, if desired.
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
法律信息
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
KlenTaq is a registered trademark of Wayne Barnes.
KlenTaq is a registered trademark of Wayne Barnes.
KlenTaq is a registered trademark of Wayne Barnes
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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