Taq DNA聚合酶 来源于水生栖热菌

了解聚合酶链反应（PCR）的历史，从促进它的发现的基本原理到获得诺贝尔化学奖，以及实时PCR（qPCR）和数字PCR等最近的发展。

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

了解标准PCR方案步骤并查看试剂清单或循环参数。此方法利用标准Taq DNA聚合酶对DNA进行常规PCR扩增。