D5921
DMEM - low glucose
With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
别名:
DME, DMEM
Product Name
杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes
运输
ambient
储存温度
2-8°C
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一般描述
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素以及其他补充组分。据报告首个原始DME配方已用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。4,500 mg/L葡萄糖的修饰对培养某些类型的细胞效果最佳。已证明DME可提高培养基的稳定性。
应用
杜氏改良Eagle低葡萄糖培养基已用于培养：
- 结肠癌SW620活细胞
- 人乳腺癌细胞系MCF-7(13)、KPL和MDA-MB-231
- 哺乳动物和犬源性脂肪源性间充质干细胞
制备说明
补充 0.584 g/L L -谷氨酰胺。
其他说明
使用干细胞或细胞克隆的研究者通常更喜欢不含酚红的培养基。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Fabian E Ortega et al.
eLife, 8 (2019-02-06)
Listeria monocytogenes hijacks host actin to promote its intracellular motility and intercellular spread. While L. monocytogenes virulence hinges on cell-to-cell spread, little is known about the dynamics of bacterial spread in epithelia at a population level. Here, we use live
Kenji Ohe et al.
The Journal of steroid biochemistry and molecular biology, 182, 21-26 (2018-04-22)
The high-mobility group A protein 1a (HMGA1a) protein is known as an oncogene whose expression level in cancer tissue correlates with the malignant potential, and known as a component of senescence-related structures connecting it to tumor suppressor networks in fibroblasts.
C Mignon et al.
Scientific reports, 7(1), 2797-2797 (2017-06-07)
Photobiomodulation-based (LLLT) therapies show tantalizing promise for treatment of skin diseases. Confidence in this approach is blighted however by lamentable inconsistency in published experimental designs, and so complicates interpretation. Here we interrogate the appropriateness of a range of previously-reported treatment
Christina Schmid et al.
Journal of pharmacological and toxicological methods, 106, 106915-106915 (2020-09-02)
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are being evaluated for their use in pharmacological and toxicological testing, particularly for electrophysiological side effects. However, little is known about the composition of the commercially available iCell cardiomyocyte (Fuijifilm Cellular Dynamics) cultures
Philip H Choi et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(35), E7226-E7235 (2017-08-16)
Cyclic di-3',5'-adenosine monophosphate (c-di-AMP) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. Our earlier studies showed that c-di-AMP regulates central metabolism in Listeria monocytogenes by inhibiting its pyruvate carboxylase (LmPC)
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