D6165
地美可辛
Hybri-Max™, powder, γ-irradiated, suitable for hybridoma
别名:
N-去乙酰-N-甲基秋水仙碱
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经验公式（希尔记法）:
C21H25NO5
化学文摘社编号:
分子量:
371.43
EC Number:
207-514-6
UNSPSC Code:
12352200
PubChem Substance ID:
Beilstein/REAXYS Number:
2822892
MDL number:
InChI key
NNJPGOLRFBJNIW-HNNXBMFYSA-N
InChI
1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1
SMILES string
CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13
grade
Hybri-Max™
sterility
γ-irradiated
form
powder
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
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Application
Recommended for use in culture medium at a final concentration of 0.4 μg/ml. Incubate cultures for 4-6 hours at 37 °C. Remove medium containing demecolcine, replace with fresh medium.
Biochem/physiol Actions
通常在核型分析和细胞周期研究中，其可在细胞周期的特定阶段增加有丝分裂细胞的产量。这可以通过多种方式来实现，其中最流行的是使用细胞周期同步剂，例如地美考辛。地美考辛会将细胞停滞于中期，同时对有丝分裂细胞或经同步的G1和S期细胞中的生化事件无明显影响。经常会用地美可辛处理白细胞以阻止中期细胞。
Preparation Note
Reconstitute 1 mg vial demecolcine with 10 mL of sterile balanced salt solution. After reconstitution, store at 2-8 °C or in frozen aliquots at −20 °C. Repeated freezing and thawing is not recommended.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
pcodes
Hazard Classifications
Acute Tox. 2 Oral
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
法规信息
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