D6421
DMEM/F-12
With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
DME/F-12, 1:1混合
产品名称
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L
运输
ambient
储存温度
2-8°C
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一般描述
由精确测量、化学成分确定的混合物构成的基础培养基，其是一种复合培养基。DMEM是一种补充有血清的培养基，有时还包含胰蛋白胨磷酸盐肉汤（TPB）成分。DMEM/F-12是一种成分完全确定的合成培养基，其不含蛋白质成分。
应用
DMEM：F12是DMEM和Ham's F12培养基的50:50混合物，其已被证明可用于广泛的细胞培养应用中，特别是当需要补充胎牛血清（FBS）时。 Ham′s Nutrient Mixture F12最初是为中国仓鼠卵巢（CHO）细胞、肺细胞和小鼠L细胞的无血清克隆生长而开发的。它经常与透析的血清、激素、硒和其他补充剂一起用于无血清培养。它是支持啮齿动物来源细胞生长的首选培养基，特别是兔和大鼠，其已被证明是骨髓瘤和杂交瘤细胞的优良克隆培养基。
适合在低血清条件下生长多种细胞类型。
杜氏′改良Eagle′s 培养基/营养混合物F-12 Ham可用于细胞培养&增殖检测。它还可用于骨髓衍生的巨噬细胞的培养。
杜氏′改良Eagle′s 培养基/营养混合物F-12 Ham可用于细胞培养&增殖检测。它还可用于骨髓衍生的巨噬细胞的培养。
制备说明
以0.365g/L补充 L-谷氨酰胺。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
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商品
Nutrient Mixture F-12 Ham Formulation
实验方案
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
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