D6421
DMEM/F-12
With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
DME/F-12, 1:1混合
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产品名称
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L
shipped in
ambient
storage temp.
2-8°C
General description
由精确测量、化学成分确定的混合物构成的基础培养基，其是一种复合培养基。DMEM是一种补充有血清的培养基，有时还包含胰蛋白胨磷酸盐肉汤（TPB）成分。DMEM/F-12是一种成分完全确定的合成培养基，其不含蛋白质成分。
Application
DMEM：F12是DMEM和Ham's F12培养基的50:50混合物，其已被证明可用于广泛的细胞培养应用中，特别是当需要补充胎牛血清（FBS）时。 Ham′s Nutrient Mixture F12最初是为中国仓鼠卵巢（CHO）细胞、肺细胞和小鼠L细胞的无血清克隆生长而开发的。它经常与透析的血清、激素、硒和其他补充剂一起用于无血清培养。它是支持啮齿动物来源细胞生长的首选培养基，特别是兔和大鼠，其已被证明是骨髓瘤和杂交瘤细胞的优良克隆培养基。
适合在低血清条件下生长多种细胞类型。
杜氏′改良Eagle′s 培养基/营养混合物F-12 Ham可用于细胞培养&增殖检测。它还可用于骨髓衍生的巨噬细胞的培养。
杜氏′改良Eagle′s 培养基/营养混合物F-12 Ham可用于细胞培养&增殖检测。它还可用于骨髓衍生的巨噬细胞的培养。
Preparation Note
以0.365g/L补充 L-谷氨酰胺。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
商品
Nutrient Mixture F-12 Ham Formulation
E Zweygarth et al.
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Yohei Ueda et al.
Scientific reports, 9(1), 8547-8547 (2019-06-14)
Growth retardation is an important side effect of glucocorticoid (GC)-based drugs, which are widely used in various preparations to treat many pediatric diseases. We investigated the therapeutic effect of exogenous CNP-53, a stable molecular form of intrinsic CNP, on a
Hrvoje Augustin et al.
PLoS biology, 15(9), e2001655-e2001655 (2017-09-14)
Lowered insulin/insulin-like growth factor (IGF) signaling (IIS) can extend healthy lifespan in worms, flies, and mice, but it can also have adverse effects (the "insulin paradox"). Chronic, moderately lowered IIS rescues age-related decline in neurotransmission through the Drosophila giant fiber
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D6421-500ML | 04061833587522 |
| D6421-24X500ML | 04061833587515 |
| D6421-6X500ML | 04061833587539 |