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Merck
CN

DNAQF

Sigma-Aldrich

DNA 定量试剂盒（荧光法）

Quantitation of DNA using bisBenzimide

别名:

dsDNA quantification

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关于此项目

UNSPSC代码:
41105500
NACRES:
NA.84

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质量水平

200

用途

sufficient for 750 assays (2 ml each)

技术

nucleic acid detection: suitable

储存温度

−20°C

一般描述

该试剂盒使用荧光染料bisBenzimide（Hoechst 33258）对DNA进行定量所需的所有试剂。该试剂盒可以高灵敏、快速、准确地测定10ng/ml至5μg/ml的双链DNA，即使存在RNA和蛋白质的情况下也是如此。荧光测定法是一种灵敏度高、简单易行的DNA定量方法。荧光染料bisBenzimide（Hoechst 33258）主要结合双链DNA（dsDNA）小沟内的AT序列，专门用于量化DNA的毫微克量（10 ng/ml至10 mg/ml）。当在360 nm激发时，DNA环境下染料在460 nm处的荧光发射显著增加。

应用

DNA定量试剂盒，荧光法已用于测量DNA浓度/含量：
  • 新生大鼠心室肌细胞(NRVM)
  • 在人间充质干细胞(hMSC)/样品中，进行相对碱性磷酸酶(ALP)量标准化
  • 小鼠胚胎成纤维细胞（NIH3T3细胞）

特点和优势

  • 专用于ng级别DNA的定量
  • 适用于纯化制备物，和纯化含有RNA和蛋白质的粗提取物中的DNA

仅试剂盒组分

产品编号
说明
  • Detailed technical bulletin 1 ea

储存分类代码

10 - Combustible liquids

法规信息

低风险生物材料
动植物源性产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000442289
0000442289
0000242664
0000242664
0000177622

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. I see that one of the componets of Product DNAQF, DNA Quantitation Kit, Fluorescence Assay, is a DNA standard. How is the DNA standard prepared? Can it be purchased separately?  

    The DNA standard (Product No. D4810) used in the kit is a sheared calf thymus DNA. It is the only one tested with the kit and it is only available in the kit. We do not determine the molecular weight.

  6. How can I ensure accurate initial quantification of the DNA standard by spectrophotometric measurement at A260 when using Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    The DNA standard (Product No. D4810) should be incubated at 50 °C for 15-30 minutes before determining the concentration spectrophotometrically. This will ensure complete dissolution of the genomic DNA.

  7. What quality of genomic DNA is required for use in Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    Genomic DNA purified by phenol chloroform extraction is sufficiently clean for use with this kit.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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This study evaluated the effect of pulsed low-intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by human dermal fibroblasts encapsulated in alginate. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content, dimethylmethylene blue assay for glycosaminoglycan
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The present study was designed to afford a critical review of the effect of proteins on the Hoechst 33258 method for determination of DNA of crude homogenates. A considerable effect of proteins on the fluorescence was observed when the concentration
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