Quality Level
usage
sufficient for 750 assays (2 ml each)
technique(s)
nucleic acid detection: suitable
storage temp.
−20°C
General description
该试剂盒使用荧光染料bisBenzimide（Hoechst 33258）对DNA进行定量所需的所有试剂。该试剂盒可以高灵敏、快速、准确地测定10ng/ml至5μg/ml的双链DNA，即使存在RNA和蛋白质的情况下也是如此。荧光测定法是一种灵敏度高、简单易行的DNA定量方法。荧光染料bisBenzimide（Hoechst 33258）主要结合双链DNA（dsDNA）小沟内的AT序列，专门用于量化DNA的毫微克量（10 ng/ml至10 mg/ml）。当在360 nm激发时，DNA环境下染料在460 nm处的荧光发射显著增加。
Application
DNA定量试剂盒，荧光法已用于测量DNA浓度/含量：
- 新生大鼠心室肌细胞(NRVM)
- 在人间充质干细胞(hMSC)/样品中，进行相对碱性磷酸酶(ALP)量标准化
- 小鼠胚胎成纤维细胞（NIH3T3细胞）
Features and Benefits
- 专用于ng级别DNA的定量
- 适用于纯化制备物，和纯化含有RNA和蛋白质的粗提取物中的DNA
仅试剂盒组分
产品编号
说明
- Detailed technical bulletin 1 ea
存储类别
10 - Combustible liquids
法规信息
低风险生物材料
动植物源性产品
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| 货号 | GTIN |
|---|---|
| DNAQF-1KT | 04061833591680 |
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