生物来源
human
生长模式
Adherent
核型
2n=46
形态学
elongated/polarized, neuronal
相关疾病
Parkinson′s disease
运输
dry ice
储存温度
−196°C
应用
These products provide unique and versatile tools for the study of central nervous system (CNS) disorders, such as Alzheimer′s disease (AD), Parkinson′s disease (PD), autism, schizophrenia, and amyotrophic lateral sclerosis (ALS), as well as for drug screening and toxicology applications in the neural space.
生化/生理作用
biallelic knock-out of PARK7 gene
特点和优势
This product contains cryo-preserved, pre-differentiated dopaminergic neurons derived from a footprint-free, karyotype normal human iPSC line with a homozygous knockout in PARK7 made using zinc fingers. It is designed for customers to generate mature dopaminergic neurons using the optimized maturation medium and supplements (DOPA1050, DOPA1051 sold separately). Mature and functional dopaminergic neurons can be obtained within 12-14 days. The dopaminergic precursors can be seeded on various culture vessel formats including 96-well plates on either glass or plastic surfaces and cultured as adherent cells. Shortly after seeding, the cells proliferate slightly for up to 4 days and show extensive neurite outgrowth and proper neuronal morphology. In general, at 12-14 days post-seeding, the cell population will contain >80% neurons, >30% TH+ dopaminergic neurons and < 15% GFAP+ astrocytes.
制备说明
No subculture routine is necessary. See technical bulletin for additional details.
User must also purchase Dopa Maturation Medium, DOPA1050 (50mL) or DOPA1051 (250mL). Sold separately.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
Andrzej Swistowski et al.
Stem cells (Dayton, Ohio), 28(10), 1893-1904 (2010-08-18)
Human induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells represent a promising unlimited cell source for generating patient-specific cells for biomedical research and personalized medicine. As a first step, critical to clinical applications, we attempted to develop defined culture
Yiping Yan et al.
Stem cells translational medicine, 2(11), 862-870 (2013-10-12)
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, are unique cell sources for disease modeling, drug discovery screens, and cell therapy applications. The first step in producing neural lineages from hPSCs is
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