mol wt
28.93 kDa
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Application
在分离DNA和RNA的过程中,对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中,用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
Biochem/physiol Actions
蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族,具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定:pH,缓冲盐,去污剂(SDS)以及温度。 在0.1-0.5% SDS存在的情况下,蛋白酶K可保持活性,在不损害分离DNA完整性的前提下,可以消化DNA制剂中的多种蛋白质和核酸酶。
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Elucidation of the structure of PrP(Sc) continues to be one major challenge in prion research. The mechanism of propagation of these infectious agents will not be understood until their structure is solved. Given that high resolution techniques such as NMR
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The aggregation of α-synuclein into amyloid fibrils constitutes a key step in the onset of Parkinson's disease. Amyloid fibrils of α-synuclein are the major component of Lewy bodies, histological hallmarks of the disease. Little is known about the mechanism of
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The Journal of antibiotics, 66(2), 55-60 (2012-11-15)
The dominant role of biofilm-associated protein (Bap) in Staphylococcus aureus biofilm development prompted us to investigate Bap as a potential target for proteinase-mediated biofilm dispersion. Biofilm assay in microtitre plates showed that proteinase K hampered the early adhesion of cells
Enzymatic sculpting of nanoscale and microscale surface topographies.
Jen-Huang Huang et al.
Angewandte Chemie (International ed. in English), 51(38), 9619-9623 (2012-08-29)
Xinping Lin et al.
Yeast (Chichester, England), 29(11), 467-474 (2012-10-16)
Red yeasts hold great promise in the production of microbial lipids and carotenoids. Genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. Here a highly efficient method for
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