E0409
Monoclonal Anti-EDEM3 antibody produced in mouse
~1.0 mg/mL, clone EDEM3-1, purified immunoglobulin, buffered aqueous solution
别名:
Anti-ER degradation enhancer, mannosidase α-like 3
产品名称
Monoclonal Anti-EDEM3 antibody produced in mouse, ~1.0 mg/mL, clone EDEM3-1, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
EDEM3-1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~120 kDa
species reactivity
rat, mouse, human
concentration
~1.0 mg/mL
technique(s)
western blot: 1-2 μg/mL using whole extract of mouse 3T3 or rat NRK cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... EDEM3(80267)
mouse ... Edem3(66967)
rat ... Edem3(289085)
Application
Monoclonal Anti-EDEM3 antibody produced in mouse has been used in immunoblotting.
Biochem/physiol Actions
EDEM3 (ER degradation enhancer, mannosidase α-like 3), a soluble EDEM homolog, enhances glycoprotein endoplasmic reticulum-associated degradation (ERAD) and mannose trimming. EDEM3 accelerates ERAD of misfolded glycoproteins as well, but in ontrast to EDEM1, it greatly stimulates mannosidase trimming in vivo.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-EDEM3 (mouse IgG1 isotype) is derived from the hybridoma EDEM3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EDEM3 conjugated to KLH.
Three EDEM homologs, EDEM1, EDEM2 and EDEM3 have been identified, which are transcriptionally upregulated upon ER stress by the activated IRE1/Xbp-1 branch.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation
van der Goot AT, et al.
Molecular Cell, 70(3), 516-530 e6-516-530 e6 (2018)
Ginto George et al.
eLife, 10 (2021-10-27)
Sequential mannose trimming of N-glycan, from M9 to M8B and then to oligosaccharides exposing the α1,6-linked mannosyl residue (M7A, M6, and M5), facilitates endoplasmic reticulum-associated degradation of misfolded glycoproteins (gpERAD). We previously showed that EDEM2 stably disulfide-bonded to the thioredoxin
Taiki Kuribara et al.
Chembiochem : a European journal of chemical biology, 18(11), 1027-1035 (2017-04-04)
Within the endoplasmic reticulum, immature glycoproteins are sorted into secretion and degradation pathways through the sequential trimming of mannose residues from Man9 GlcNAc2 to Man5 GlcNAc2 by the combined actions of assorted α-1,2-mannosidases. It has been speculated that specific glycoforms
Min Ni et al.
FEBS letters, 581(19), 3641-3651 (2007-05-08)
The field of endoplasmic reticulum (ER) stress in mammalian cells has expanded rapidly during the past decade, contributing to understanding of the molecular pathways that allow cells to adapt to perturbations in ER homeostasis. One major mechanism is mediated by
Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens.
Richard T Timms et al.
Nature communications, 7, 11786-11786 (2016-06-11)
The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied
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