E0526
Endonuclease III from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, buffered aqueous glycerol solution, ≥10,000 units/mg protein
别名:
Nth protein, Endo III
生物来源
Escherichia coli
质量水平
重组
expressed in E. coli overproducing strain
方案
≥90% (SDS-PAGE)
表单
buffered aqueous glycerol solution
比活
≥10,000 units/mg protein
分子量
≥23 kDa (211 amino acids)
技术
activity assay: suitable
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
Escherichia coli K12 ... nth(947122)
生化/生理作用
Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme.
Endo III, product of the nth gene, is an iron-sulfur containing DNA repair enzyme. It possesses DNA glycosylase activity with broad substrate specificity for mutated pyrimidine derivatives especially thymine glycol (Tg) but also for 5-hydroxycytosine and 5,6-dihydrothymine. In addition, it has an apurinic/apyrimidinic (AP) lyase activity which cleaves the DNA backbone by β elimination. Endo III protects E. coli cells from radiation damage caused by x-ray and UV irradiation and its activity overlaps the activity of endonuclease VIII. When overexpressed, it can also protect E.coli cells from alkylation defects caused by methylmethanesulphonate.
外形
Solution in 20 mM Tris, pH 7.4, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% glycerol.
制备说明
Purified in an iron bound form that confers the protein a light green color.
其他说明
One unit will cleave 50% of 0.5 pmol double strand oligonucleotide substrate containing 5,6-dihydrothymine in 15 min. at 30 °C.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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