E2F Transcription Factor 6 human

E2F6 (E2F transcription factor 6) belongs to a family of eight transcription factors (E2F1-E2F8), called E2F. This family is divided into three groups, and E2F6, E2F7 and E2F8 belong to the third group. Its C-terminal lacks the pRb-binding domain, its N-terminal lacks the homology domain found in E2F1 to E2F3, and it also does not contain the transactivation domain found in E2F1 to E2F5. It acts as a polycomb group (PcG) transcriptional repressive complex-association protein. In humans, it is expressed in CD34⁺ hematopoietic progenitor cells.

E2F6 (E2F transcription factor 6) acts as a transcriptional repressor in a pocket-protein independent manner, and it is not transactivated and does not contain pocket-protein binding domains. Human parvovirus B19 (B19V) elevates E2F6 expression, thus, prevents cell division of human erythroid progenitors. HPV (human papilloma virus)16 E7 oncoprotein interacts with E2F6 and leads to the deregulation of its expression. It acts as an antagonist of E2F-1, which is pro-apoptotic in nature, and prevents the apoptosis of hematopoietic progenitor cells during proliferation. It interacts with BRCA1, through its C-terminal and suppresses the ultraviolet-induced apoptosis.

E2F6 is a member of the E2F family of transcription factors that play an important role in the regulation of cell cycle progression. In normal cells, E2F activity is regulated by binding to pRB, the product of the retinoblastoma gene, and by binding to "pocket proteins", p107 and p130.

Solution in 50 mM Tris, pH 7 (25 °C), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, and 30% (w/v) glycerol.

The product may appear as a triplet in SDS-PAGE.