biological source
mouse
recombinant
expressed in NSO cells
assay
>90% (SDS-PAGE)
form
lyophilized powder
potency
0.16-10 ng/mL
mol wt
monomer calculated mol wt 85.7 kDa, ~110 kDa by SDS-PAGE (reducing)
storage condition
avoid repeated freeze/thaw cycles
technique(s)
ligand binding assay: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Epha7(13841)
General description
EphA7 (ephrin receptor A7) is a receptor tyrosine kinase (RTK), which belongs to a 16 member family of ephrin receptors. This family is divided into two classes A and B. EphA5 belongs to EphA group, which contains 10 members. The ligands to this group of receptors are glycosylphosphatidylinositol (GPI)-anchored named ephrin-A1-A6. The members of this family have an ephrin-binding domain in its N-terminal globular domain, succeeded by a linker region rich in cysteine and two fibronectin type III repeats in the exoplasmic domain. Its cytoplasmic domain consists of a juxtamembrane region, a conserved tyrosine kinase domain, an α-helical C-terminal domain, and a PDZ domain-binding domian.
Biochem/physiol Actions
EphA7 (ephrin receptor A7) promotes normal synaptic functioning, and development of cortical dendrites and maturation of the spine, and acts as a guidance receptor during development and patterning of cortex. During initial stages of brain development, this receptor modulates cell apoptosis. It forms a multi-protein complex with tumor necrosis factor receptor 1 (TNFR1) and EphA5 to induce caspase-mediated cell death. In mice, it is induced by Dlx5 and Msx2, which are homeodomain-containing transcription factors. It therefore, regulates the morphogenesis of cranial neural tube, by controlling the cell adhesion. In mice, it also regulates the contouring of corticothalamic (CT) mapping. It plays the role of tumor-suppression in follicular lymphoma (FL), and thus has potential as a therapeutic target.
Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, and ephrin-A5; involved in pattern formation and morphogenesis.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Analysis Note
The biological activity is measured by its ability to bind recombinant human ephrin-A4/Fc in an ELISA assay.
Other Notes
Extracellular domain of mouse EphA7 (amino acids 1-549) fused by means of a polypeptide linker to the Fc region of human IgG1 that is histidine-tagged at the C-terminus.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Meredith A Clifford et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(13), 4994-4999 (2014-04-08)
The process by which excitatory neurons are generated and mature during the development of the cerebral cortex occurs in a stereotyped manner; coordinated neuronal birth, migration, and differentiation during embryonic and early postnatal life are prerequisites for selective synaptic connections
Haeryung Lee et al.
Molecules and cells, 35(5), 450-455 (2013-05-10)
A previous study showed that the EphA7 receptor regulates apoptotic cell death during early brain development. In this study, we provide evidence that the EphA7 receptor interacts with death receptors such as tumor necrosis factor receptor 1 (TNFR1) to decrease
Jangwoo Lee et al.
Development, growth & differentiation, 55(3), 341-349 (2013-02-22)
Homeodomain-containing transcription factors Dlx5 and Msx2 are able to form a heterodimer, and together can regulate embryonic development including skeletogenesis. Dlx5 functions as a transcriptional activator and Msx2 a transcriptional repressor, and they share common target genes. During mouse digit
Sandra Pietri et al.
Cerebral cortex (New York, N.Y. : 1991), 22(7), 1678-1689 (2011-09-24)
The patterning of cortical areas is controlled by a combination of intrinsic factors that are expressed in the cortex and external signals such as inputs from the thalamus. EphA7 is a guidance receptor that is involved in key aspects of
Elisa Oricchio et al.
Cell, 147(3), 554-564 (2011-11-01)
Insights into cancer genetics can lead to therapeutic opportunities. By cross-referencing chromosomal changes with an unbiased genetic screen we identify the ephrin receptor A7 (EPHA7) as a tumor suppressor in follicular lymphoma (FL). EPHA7 is a target of 6q deletions
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