tag
FLAG® tagged, c-Myc tagged (C-terminal)
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
Application
The pFLAG-Myc-CMV™-19 Expression Vector (4.7 kb) is used to establish transient expression of secreted, dual-tagged N-terminal FLAG® and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes the FLAG® epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively.
Expression vector and control vector sequences can be found here.
Expression vector and control vector sequences can be found here.
Other Notes
Browse additional application references in our FLAG® Literature portal.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
法规信息
新产品
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相关内容
p3xFLAG-Myc-CMV-19 and p3xFLAG-Myc-CMV-20 Vector Maps
Instructions
A M Campbell et al.
The Journal of biological chemistry, 267(13), 9321-9325 (1992-05-05)
The sarcoplasmic/endoplasmic reticulum slow-twitch or cardiac Ca(2+)-ATPase (SERCA2) is expressed as two forms (SERCA2a and SERCA2b) which vary at their extreme carboxyl termini. SERCA2a and SERCA2b are derived from alternatively spliced primary transcripts of the same gene. These two alternative
S Andersson et al.
The Journal of biological chemistry, 264(14), 8222-8229 (1989-05-15)
The conversion of cholesterol into bile acids in the liver represents the major catabolic pathway for the removal of cholesterol from the body. In this complex biosynthetic pathway, at least 10 enzymes modify both the ring structure and side chain