biological source
human
Quality Level
recombinant
expressed in Saccharomyces cerevisiae
form
liquid
specific activity
≥5 units/pmol enzyme (P450 activity)
secondary activity
≥500 units/mg protein (reductase activity)
concentration
≥10 mg/mL protein (Bradford), 1 pmol/μL
UniProt accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYP2E1(1571)
General description
Microsome contains CYP2E1 human and yeast CYP-reductase expressed in Saccharomyces cerevisiae.
Application
Recombinant cytochrome P450, isozyme 2E1, (CYP2E1) may be used in toxicity and drug metabolism/pharmacokinetics research to identify its specificity and activity versus xenobiotic and endogenous compounds. Recombinant cytochrome P450, isozyme 2E1, may be used separately or as a component of a P450 isozyme panel to identify and characterize natural and synthetic selective inhibitors that affect the metabolism of toxic compounds or therapeutic drugs.
Physical form
50 mM Tris (pH 7,4), 1 mM EDTA, 20% glycerol
Other Notes
P450 Activity: One unit will oxidize 1 picomole of p-nitrophenol per minute at pH 7.4 at 28 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome C per minute in the presence of NADPH at pH 7.4 at 28 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome C per minute in the presence of NADPH at pH 7.4 at 28 °C.
For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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