biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HK19, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
flow cytometry: 10 μL using 1 × 106 cells
isotype
IgG2b
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... HLA-DQB1(3119)
General description
HLA-DQ molecules are bimolecular glycoprotein complexes of 29 kDa and 33 kDa components. The HLA-DQ antigens are present on peripheral blood lymphocytes and tonsillar and splenic mononuclear cells.
Immunogen
GM-1500 human tumor cell line.
Biochem/physiol Actions
Recognizes MHC Class II molecules that are preferentially expressed on the surface of B lymphocytes. The antibody reacts weakly with resting or ConA-activated T cells, monocytes, granulocytes, and erythrocytes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
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M Mahmoudi-Rad et al.
Clinical and experimental dermatology, 38(6), 646-651 (2013-07-11)
Various materials have been investigated as possible skin substitutes to repair skin defects such as burns. Because of its unique characteristics, acellular amniotic membrane seems to provide a good scaffold for cell cultures. To investigate the proliferation of fibroblasts on
H Moravvej et al.
The British journal of dermatology, 179(1), 72-79 (2018-01-14)
Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB). To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with those of fibroblasts seeded on amniotic membrane scaffolds (FAMS)
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