生物来源
mouse
重组
expressed in E. coli
方案
>97% (SDS-PAGE)
表单
lyophilized powder
效能
10-50 ng/mL ED50
分子量
apparent mol wt 31 kDa by SDS-PAGE
predicted mol wt 28 kDa
包装
pkg of 25 μg
储存条件
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
杂质
endotoxin, tested
UniProt登记号
储存温度
−20°C
基因信息
mouse ... Fgf8(14179)
一般描述
Fgf8 (fibroblast growth factor 8) is a member of the FGF family of proteins. This family was originally identified as fibroblast mitogen in bovine pituitary and brain. Both humans and mice, have six to seven subfamilies of FGF protein containing 22 members. FGF gene is composed of three exons, and the first exon is alternatively spliced to produce four variants. This gene is expressed in embryonic organs such as brain, eye, ear, hearts and limbs.
应用
Fibroblast Growth Factor-8c from mouse is suitable for the prevention of cross-reaction in Western blot and SDS-PAGE for FGF2 protein.
生化/生理作用
FGF-8c plays a role in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system.
Fgf8 (fibroblast growth factor 8) plays an essential role in the development of dorsal raphe nucleus (DR), and is expressed in fetus in the developing anterior hindbrain. DR serotonergic neurons are born in this area of the brain, and this is achieved due to the formation of diffusion gradient by the secreted Fgf8 peptide. Failure of this results in loss of serotonergic neurons during brain development. A nonsense mutation in this gene is linked with isolated hypogonadotropic hypogonadism (IHH), with different degrees of olfactory phenotype and human gonadotropin-releasing deficiency.
Plays a role in ectodermal differentiation in the post-gastrulation embryo.
外形
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 1.25 mg bovine serum albumin.
分析说明
The biological activty is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
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FGFR1 mutations cause isolated hypogonadotropic hypogonadism (IHH) with or without olfactory abnormalities, Kallmann syndrome, and normosmic IHH respectively. Recently, missense mutations in FGF8, a key ligand for fibroblast growth factor receptor (FGFR) 1 in the ontogenesis of GnRH, were identified
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