biological source
mouse
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
10-50 ng/mL ED50
mol wt
apparent mol wt 31 kDa by SDS-PAGE, predicted mol wt 28 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Fgf8(14179)
General description
Fgf8 (fibroblast growth factor 8) is a member of the FGF family of proteins. This family was originally identified as fibroblast mitogen in bovine pituitary and brain. Both humans and mice, have six to seven subfamilies of FGF protein containing 22 members. FGF gene is composed of three exons, and the first exon is alternatively spliced to produce four variants. This gene is expressed in embryonic organs such as brain, eye, ear, hearts and limbs.
Application
Fibroblast Growth Factor-8c from mouse is suitable for the prevention of cross-reaction in Western blot and SDS-PAGE for FGF2 protein.
Biochem/physiol Actions
FGF-8c plays a role in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system.
Fgf8 (fibroblast growth factor 8) plays an essential role in the development of dorsal raphe nucleus (DR), and is expressed in fetus in the developing anterior hindbrain. DR serotonergic neurons are born in this area of the brain, and this is achieved due to the formation of diffusion gradient by the secreted Fgf8 peptide. Failure of this results in loss of serotonergic neurons during brain development. A nonsense mutation in this gene is linked with isolated hypogonadotropic hypogonadism (IHH), with different degrees of olfactory phenotype and human gonadotropin-releasing deficiency.
Plays a role in ectodermal differentiation in the post-gastrulation embryo.
Physical form
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 1.25 mg bovine serum albumin.
Analysis Note
The biological activty is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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Bettina A Moser et al.
The EMBO journal, 28(7), 810-820 (2009-02-14)
To maintain genomic integrity, telomeres must undergo switches from a protected state to an accessible state that allows telomerase recruitment. To better understand how telomere accessibility is regulated in fission yeast, we analysed cell cycle-dependent recruitment of telomere-specific proteins (telomerase
Ericka B Trarbach et al.
The Journal of clinical endocrinology and metabolism, 95(7), 3491-3496 (2010-05-14)
FGFR1 mutations cause isolated hypogonadotropic hypogonadism (IHH) with or without olfactory abnormalities, Kallmann syndrome, and normosmic IHH respectively. Recently, missense mutations in FGF8, a key ligand for fibroblast growth factor receptor (FGFR) 1 in the ontogenesis of GnRH, were identified
X Shi et al.
Oncogenesis, 4, e176-e176 (2015-12-01)
Sonic hedgehog (Shh) signaling is critical during normal development, and the abnormal activation of the Shh pathway is involved in many human cancers. As a target gene of the Shh pathway and as a transcription activator downstream of Shh signaling
N Abimbola Sunmonu et al.
Journal of cellular physiology, 226(7), 1722-1726 (2011-04-21)
Soluble growth factors play an important role in the coordination and integration of cell proliferation, differentiation, fate determination, and morphogenesis during development of multicellular organisms. Fibroblast growth factors (FGFs) are a large family of polypeptide growth factors that are present
Tetsu M C Yung et al.
Experimental cell research, 315(10), 1693-1705 (2009-02-28)
Negative Elongation Factor (NELF) is a transcription factor discovered based on its biochemical activity to suppress transcription elongation, and has since been implicated in various diseases ranging from neurological disorders to cancer. Besides its role in promoter-proximal pausing of RNA
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