Monoclonal Anti-hFBXW7γ 小鼠抗

FBXW7 is a F-box protein that facilitates as a substrate recognition component of the ubiquitin ligase complex SCF (Skp-Cullin-F-box). FBXW7 exist in three isoforms α, β and γ. It is composed of a conserved F-box domain at their N terminus, which interacts with Skp1. C terminus consist of one or several highly variable protein-protein interaction domains such as Leu-rich repeat (LRR), kelch repeat, tetratricopeptide repeat (TPR), and WD40 repeat.

synthetic peptide corresponding to amino-acids 13-27 of human hFBXW7γ isoform.

Monoclonal Anti-hFBXW7γ antibody is suitable for:-

• Immunocytochemistry at a concentration of 10-20μg/mL using human A431 cells.
• Western blot at a concentration of 5-10μg/mL using human A431 cell extracts.
• Indirect ELISA.

FBXW7 protein is highly involved in the ubiquitination of cyclin E , Notch , c-Jun and c-Myc. It acts as a key component of phosphoepitope-specific substrate recognition complex of SCF ubiquitin ligases to facilitate the recognition of phosphorylated targets such as cyclin E, myc, c-Jun, and Notch. The SV40 large T antigen binds FBXW7 without detectible effects on its stability to acts as an inhibitor of FBXW7 activity.It has been reported that FBXW7 controls c-Myc′s growth-promoting activity followed by cell size, which is an important event of Fbw7 loss in cancers.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

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