F2258
焦磷酸依赖性果糖-6-磷酸激酶 来源于马铃薯块茎
lyophilized powder, ≥2 units/mg protein (Lowry)
别名:
6-磷酸果糖激酶（焦磷酸盐）, 焦磷酸盐: D-果糖-6-磷酸 1-磷酸转移酶
form
lyophilized powder
specific activity
≥2 units/mg protein (Lowry)
composition
Protein, 20-40% Lowry
storage temp.
−20°C
Biochem/physiol Actions
Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.
Physical form
含磷酸钠缓冲盐和稳定剂以及微量无机焦磷酸盐和二硫苏糖醇的冻干粉
Other Notes
在 pH 8.0 和 30°C 以及 1μM 果糖-2,6-二磷酸和 17mM 葡萄糖-6-磷酸存在的条件下，一单位每分钟可将 1.0μmol 焦磷酸盐和果糖-6-磷酸转化为果糖-1,6-二磷酸和无机磷酸盐。
这两种焦磷酸依赖性果糖-6-磷酸激酶的区别在于它们对生理活化剂果糖 2,6-二磷酸 [Fru (2,6)-P2]的反应不同。据报道，来自费氏丙酸杆菌的酶 (F8381) 不受 Fru (2,6)-P2 影响。而来自马铃薯块茎的酶 (F2258) 则可被 Fru (2,6)-P2 激活（在 5.5nM Fru (2,6)-P2 条件下，半最大活化马铃薯块茎酶）。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
M E Theodorou et al.
Planta, 213(1), 147-157 (2001-08-29)
A major problem in defining the physiological role of pyrophosphate:fructose 6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) is the 1,000-fold discrepancy between the apparent affinity of PFP for its activator, fructose 2,6-bisphosphate (Fru-2,6-P2), determined under optimum conditions in vitro and the estimated
Identification and cloning of the gene encoding pyrophosphate-dependent 6-phosphofructokinase of Methylomonas methanica.
A S Reshetnikov et al.
Doklady. Biochemistry and biophysics, 405, 468-470 (2006-02-17)
Guillaume Meurice et al.
In silico biology, 4(4), 517-528 (2004-10-28)
We performed a detailed bioinformatic study of the catalytic step of fructose-6-phosphate phosphorylation in glycolysis based on the raw genomic draft of Propionibacterium freudenreichii subsp. shermanii (P. shermanii) ATCC9614 [Meurice et al., 2004]. Our results provide the first in silico
J Suzuki et al.
Genetics and molecular research : GMR, 2(4), 376-382 (2004-03-11)
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have
Fabrício Ramon Lopes et al.
Molecular genetics and genomics : MGG, 279(4), 385-401 (2008-01-31)
Transposable elements are major components of plant genomes and they influence their evolution, acting as recombination hot spots, acquiring specific cell functions or becoming part of protein-coding regions. The latter is the subject of the present analysis. This study is
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