Anti-FKHR (FOXO1a) antibody produced in rabbit

Forkhead box protein O1 (FOXO1a) belongs to the forkhead box transcription factor (FOXO) O class family. It comprises of forkhead domain nuclear localization motif, nuclear export and transactivation motifs. FOXO1 gene is mapped to human chromosome 13q14.11.

synthetic peptide corresponding to human FKHR (amino acids 636-651).

Anti-FKHR (FOXO1a) antibody produced in rabbit may be used in immunoblotting.

Anti-FKHR (FOXO1a) recognizes human and mouse FKHR (FOXO1a).

Forkhead family members have been shown to play key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. Forkhead box protein O1 (FOXO1a) or Forkhead in rhabdomyosarcoma (FKHR) contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB). Likewise, induction by insulin-like growth factor (IGF-1) and insulin leads to phosphorylation of one or more of these sites. In addition to the involvement of FKHR in apoptosis, it was shown to be involved in glucose homeostasis, cell cycle regulation, and as a nuclear receptor cofactor. FOXO1a has been identified at chromosomal breakpoints in human tumors especially leukemia and rhabdomyosarcoma. FKHR is downregulated in endometrial carcinoma. Antibodies reacting specifically with FKHR (FOXO1a) may be useful in studying the expression and function of the protein, as well as in correlating its expression pattern with physiological functions or pathological conditions.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.

For continuous use, store at 2–8 °C for up to one month. For prolonged storage, freeze in working aliquots at 20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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