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Merck
CN

F2653

Sigma-Aldrich

抗-小鼠IgG (Fab特异性) F(ab′)2片段–FITC 山羊抗

affinity isolated antibody, buffered aqueous solution

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关于此项目

MDL编号:
MFCD00162622
UNSPSC代码:
12352203
NACRES:
NA.46

主要文件

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生物来源

goat

偶联物

FITC conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

技术

flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

储存温度

−20°C

靶向翻译后修饰

unmodified

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相关类别

一般描述

IgG是一种单体，是血液中循环的主要抗体，可帮助吞噬破坏外来微生物，并且是人类中唯一可以穿过胎盘的Ig。通过抗小鼠IgG（Fab特异性）-FITC抗体的差异结合进行细胞分选，可将肿瘤细胞与巨噬细胞分离。该抗体可用于孵育体细胞核移植（SCNT）大鼠胚胎进行免疫细胞化学观测值。 可作为二抗用于研究小肠、派尔集合淋巴结生发中心、非生发中心、脾脏和肠系膜淋巴结中活化B淋巴细胞的动态变化。抗小鼠IgG抗体与全部小鼠免疫球蛋白发生特异性反应。
免疫球蛋白G（IgG）属于免疫球蛋白家族，是一种广泛表达的血清抗体。免疫球蛋白是由两条重链和两条轻链通过二硫键连接而成的。它主要用于帮助免疫防御。它是一种糖蛋白。IgG是一种主要的免疫球蛋白。小鼠具有五种类型的免疫球蛋白 - IgM，IgG，IgA，IgD和IgE。小鼠IgG进一步分为五类 - IgG1、IgG2a、IgG2b和IgG3。

免疫原

小鼠IgG的纯化Fab片段。

应用

成功使用该抗体的应用以及相关同行评审论文如下所示。
流式细胞术/细胞分选（1篇论文）
抗小鼠IgG（Fab特异性）FITC偶联抗体用于ZAP-70表达的传统免疫细胞化学评估方法。它也可以用于SCNT胚胎的免疫细胞化学观测值。
羊抗鼠IgG（Fab特异性）F（ab′）2片段FITC抗体用于：
  • 间接免疫荧光染色免疫
  • 荧光染色
  • 流式细胞仪

生化/生理作用

免疫球蛋白G（IgG）参与II型和III型超敏反应。IgG有助于调理素作用、补体结合和抗体依赖型细胞介导的细胞毒性作用。
可与所有小鼠Igs结合。
可用于尝试避免因Fc受体存在而引起的背景染色。

外形

溶于含有1%牛血清白蛋白和15 mM叠氮化钠的0.01M磷酸盐缓冲生理盐水（pH 7.4）的溶液。

制备说明

吸附以减少牛、马或人样本的背景染色。

其他说明

经过牛、马和人血清蛋白吸附的抗体。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000497436
0000497436
0000485822
0000485822
0000274313

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What applications have product F2653, Anti-Mouse IgG (Fab specific) F(ab´)2 fragment-FITC antibody produced in goat, been used in?

    Product F2653, Anti-Mouse IgG (Fab specific) F(ab´)2 fragment-FITC antibody produced in goat,  has been used in Flow Cytometry in the following literature references.Bergman, I. et al., Clinical Cancer Research., 7, 2050 (July 2001).Farsinejad, A. et al. Iranian Journal of Blood and Medicine, 2, Number 3, (Spring 2010).This antibody has been used in Immunofluorescencs in the following literature reference.Sushan, A., Hum. Reprod., 19 (9) : 1957-1967 (2004).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Highly tumorigenic cancer cell (HTC) populations have been identified for a variety of solid tumors and assigned stem cell properties. Strategies for identifying HTCs in solid tumors have been primarily empirical rather than rational, particularly in epithelial tumors, which are
Eabha O'Driscoll et al.
Journal of clinical medicine, 12(7) (2023-04-14)
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Mustapha Irnaten et al.
Investigative ophthalmology & visual science, 59(2), 831-842 (2018-02-08)
Optic nerve cupping in glaucoma is characterized by remodeling of the extracellular matrix (ECM) and fibrosis in the lamina cribrosa (LC). We have previously shown that glaucoma LC cells express raised levels of ECM genes and have elevated intracellular calcium
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