Anti-Focal Adhesion Kinase (pp125^FAK) antibody produced in rabbit

Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase that is expressed ubiquitously. FAK is the critical in forming cell-substratum adhesions or focal adhesion complexes. FAK reportedly has multiple phosphorylation sites. Autophosphorylation of FAK at Tyr³⁹⁷ creates a binding site on FAK for Src-family kinases and is critical for maximum adhesion and migration responses. The phosphorylation of Tyr⁴⁰⁷ and Tyr861 is induced during epithelial-mesenchymal transition and further augmented during cell migration. FAK activation couples signal transduction via PI3K, CAS and Src. FAK signaling is involved in multiple functions such as proliferation, migration, survival and angiogenesis. Disruption of FAK signalling results in failure of adhesion to the substratum and anoikis (apoptosis) of epithelial cells which are anchorage dependent. FAK has been observed to be upregulated in human tumors such as neuroblastoma and is related to tumor cell survival and prognosis
Anti-Focal Adhesion Kinase (pp125 ^FAK) antibody recognizes FAK (Focal Adhesion Kinase) in human endothelial cultured cell line (125 kDa). An additional, weaker, lower molecular weight band may be seen.

synthetic peptide corresponding to C-terminal region amino acids 1039-1052 of human, mouse, or rat focal adhesion kinase (pp125^FAK).

Anti-Focal Adhesion Kinase (pp125fak) antibody may be used at a working dilution of 1:2000 for detection by immunoblotting in human endothelial cells. The antibody is suitable for immunofluorescence at a working dilution of 1:4000. The antibody is suitable for immunoprecipitation, immunocytochemistry and protein microarray. The antibody had been used for detection by immunoblotting and immunoprecipitation in human breast cancer cell line, MCF-7.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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