F4771
抗-成纤维细胞表面蛋白单克隆抗体 小鼠抗
clone 1B10, ascites fluid
别名:
Fibroblast Surface Protein Antibody, Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
产品名称
抗-成纤维细胞表面蛋白单克隆抗体 小鼠抗, clone 1B10, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1B10, monoclonal
mol wt
antigen 43-72-80 kDa
contains
15 mM sodium azide
species reactivity
human
technique(s)
complement-mediated cytotoxicity assay: 1:500
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
工作稀释度至少为1:500，通过补体蛋白介导的微细胞毒性测定法测定。
Application
在小鼠中产生的单克隆抗成纤维细胞表面蛋白抗体可用于：
- 免疫荧光
- 流式细胞术
- 免疫组织化学
- 免疫印迹
成纤维细胞表面蛋白单克隆抗体可用于人成纤维细胞和单核细胞的流式细胞分析。此外，它还可用于免疫印迹和蛋白质印迹分析。
Biochem/physiol Actions
单克隆抗成纤维细胞表面蛋白抗体也可用于通过抑制成纤维细胞在培养容器上的附着或是通过在兔补体存在下的细胞毒性作用，从而去除培养的人细胞中的成纤维细胞。另外，除了细胞特性的重要功能外，细胞系的表征对于培养物与组织起源的相关性、细胞所属谱系的鉴定或细胞的前体状态也很重要。成纤维细胞表面蛋白的单克隆抗体可提供强大的工具，用来控制各种人类细胞培养系统中成纤维细胞的生长，例如胸腺上皮（TE）细胞培养物，从而使高度富集的TE细胞群可以生长。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途，或人类或动物任何类型的消费或使用。
General description
单克隆抗成纤维细胞表面蛋白（小鼠IgM 同种型）来源于小鼠骨髓瘤细胞和以培养的人胸腺成纤维细胞免疫的BALB/c小鼠脾细胞融合产生的1B10杂交瘤。人成纤维细胞表面蛋白是一种成纤维细胞抗原，主要在人滑膜、包皮和胸腺成纤维细胞表达，在人上皮细胞和淋巴细胞中不存在。
这种成纤维细胞抗原已经证实存在于人滑膜、乳腺、包皮和胸腺成纤维细胞以及恶性纤维肉瘤组织中。该抗原既与细胞膜又与溶酶体相关。
Immunogen
培养人胸腺成纤维细胞
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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