form
amorphous solid
specific activity
≥20 units/mg solid
mol wt
~140 kDa
shipped in
wet ice
storage temp.
−20°C
General description
等电点:5.0 ± 0.1
米氏常数:5 x 10‾3M(D-果糖)
抑制剂:Ag+,Hg++,SDS
最佳pH4.0
最佳温度:37℃
pH稳定性:pH 4.0 – 6.0(25℃,16小时)
热稳定性:低于40℃(pH 4.5,15分钟)
米氏常数:5 x 10‾3M(D-果糖)
抑制剂:Ag+,Hg++,SDS
最佳pH4.0
最佳温度:37℃
pH稳定性:pH 4.0 – 6.0(25℃,16小时)
热稳定性:低于40℃(pH 4.5,15分钟)
Application
来自葡萄糖杆菌属的D-果糖脱氢酶已用作碳水化合物结合特异性测定和SDS-PAGE(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳)中的标准品。
该酶还用于许多基础研究项目中,以检查称为生物电催化的酶催化电极反应的电化学特性。该酶可用于临床分析中D-果糖的酶促测定。
Biochem/physiol Actions
D-果糖脱氢酶是一种异源三聚体膜结合酶,常见于各种葡萄糖杆菌属,尤其在日本葡萄糖杆菌(Gluconobacter industrius)中。它的分子质量约为140 kDa,由亚基I(67kDa),II(51 kDa)和III(20 kDa)组成,催化D-果糖氧化生成5-酮-D-果糖。该酶是一种黄素蛋白-细胞色素c复合物,其亚基I和II分别与黄素腺嘌呤二核苷酸(FAD)和血红素C共价结合作为辅基。
Physical form
本品以无定形固体形式供应,含有~80%稳定剂、糖、氨基酸和BSA。
Other Notes
在37℃条件下,当pH为4.5时,一个单位每分钟将1.0 μmole D-果糖到5-酮果糖。
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
Kevin R Ramkissoon et al.
PloS one, 8(12), e84508-e84508 (2014-01-05)
The power of genome sequencing depends on the ability to understand what those genes and their proteins products actually do. The automated methods used to assign functions to putative proteins in newly sequenced organisms are limited by the size of
Rapid identification of sequences for orphan enzymes to power accurate protein annotation
Ramkissoon KR, et al.
PLoS ONE, 8(12), e84508-e84508 (2013)
Determination of seminal fructose using D-fructose dehydrogenase.
K Nakashima et al.
Clinica chimica acta; international journal of clinical chemistry, 151(3), 307-310 (1985-10-15)
Amperometric flow injection determination of fructose with an immobilized fructose 5-dehydrogenase reactor.
K Matsumoto et al.
Analytical chemistry, 58(13), 2732-2734 (1986-11-01)
J Marcinkeviciene et al.
FEBS letters, 318(1), 23-26 (1993-02-22)
Steady-state kinetic analysis was performed on the reaction between D-fructose and ferricyanide with the quinohemoprotein fructose dehydrogenase from Gluconobacter species. The D-fructose oxidation dependence on the ferricyanide concentration resulted in a series of parallel reciprocal plots, and the reaction was
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