F6131
胎球蛋白 from fetal bovine serum
γ-irradiated, BioXtra, suitable for cell culture
别名:
胎球蛋白 from fetal bovine serum, 胎球蛋白 来源于胎牛血清
sterility
γ-irradiated
product line
BioXtra
form
powder
mol wt
48.4 kDa
technique(s)
cell culture | mammalian: suitable
solubility
water: 1 mg/mL, clear to slightly hazy, colorless to faintly yellow
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... FETUB(504615)
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General description
Fetuin is a protein component of serum which contributes to the attachment and spreading of cells in culture medium. Researchers often add serum for this purpose. Fetuin has been used at a concentration of 500 μg/ml to supplement serum-free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells. Fetuin can be used as a glycoprotein standard for carbohydrate structure in a glycoprotein. The structural elucidation of the carbohydrate portion of fetuin has been published. The cDNA and amino acid sequences has been reported. Fetuin contains 12 Cys residues, all involved in disulfide bridges.
The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.
The composition of bovine fetuin (weight %) is polypeptide 74%, hexose 8.3%, hexosamines 5.5%, and sialic acid 8.7%.1 This product usually assays at 5-8% total sialic acid. Nearly all of this sialic acid is bound. The amount of free sialic acid is typically less than 0.3%. A method for determining free sialic acid content has been published.
Analysis Note
This product is cell culture tested (0.5 g/L) and is appropriate for use in cell culture applications.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Mito Kanatsu-Shinohara et al.
Biology of reproduction, 84(1), 97-105 (2010-09-17)
Spermatogonial stem cells (SSCs) undergo self-renewal divisions to support spermatogenesis. Although several in vitro SSC culture systems have been developed, these systems include serum or fibroblast feeders, which complicate SSC self-renewal analyses. Here, we developed a serum- and feeder-free culture
Ultracentrifugal and electrophoretic studies on fetuin.
K O PEDERSEN
The Journal of physical and colloid chemistry, 51(1), 164-171 (1947-01-01)
Bianca A Silva et al.
FEMS microbiology letters, 295(2), 274-280 (2009-05-23)
Herpetomonas megaseliae is a monoxenic trypanosomatid isolated from the phorid fly Megaselia scalaris. In the present report, the expression of cell surface sialoglycoconjugates in this parasite was analyzed by Western blotting, flow cytometry and fluorescence microscopy analyses using lectins that
Ana Planinc et al.
Analytical and bioanalytical chemistry, 409(2), 477-485 (2016-06-12)
Therapeutic proteins are among the top selling drugs in the pharmaceutical industry. More than 60 % of the approved therapeutic proteins are glycosylated. Nowadays, it is well accepted that changes in glycosylation may affect the safety and the efficacy of the
Anna Hultberg et al.
PloS one, 6(4), e17665-e17665 (2011-04-13)
For efficient prevention of viral infections and cross protection, simultaneous targeting of multiple viral epitopes is a powerful strategy. Llama heavy chain antibody fragments (VHH) against the trimeric envelope proteins of Respiratory Syncytial Virus (Fusion protein), Rabies virus (Glycoprotein) and
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