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Merck
CN

F7305

胎牛血清

Mouse Embryonic Stem Cell Screened, suitable for cell culture, sterile-filtered, USDA Approved

别名:

FBS, FCS, 血清

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关于此项目

UNSPSC Code:
12352207
MDL number:
Biological source:
bovine fetus
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source

bovine fetus

sterility

sterile-filtered

quality

USDA Approved

composition

Hemoglobin, ≤25 mg/dL

technique(s)

cell culture | mammalian: suitable

impurities

≤5 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting balanced growth-promoting and growth-inhibiting functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.
Most Fetal Bovine Serum (FBS) batches do not support mouse ES cells. Sigma-Aldrich has pre-screened multiple FBS batches to increase your chances of selecting a batch that will perform within your laboratory, saving you time and money.
In addition to our extensive Fetal Bovine Serum performance and quality assays (mycoplasma, sterility, endotoxin, hemoglobin, etc.), we have developed low density, feeder-free assays to assess Fetal Bovine Serum′s support of mouse embryonic stem cells. Only those batches that performed well with mouse ES cells were selected for this product.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Analysis Note

  • 内毒素和血红蛋白测试
  • Mouse embryonic stem cell screened
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions



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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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分析证书(COA)

Lot/Batch Number

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Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Alternatives to the use of fetal bovine serum: serum-free cell culture.
Gstraunthaler G
ALTEX, 20, 275-275 (2003)
Robert C Tuckey et al.
The Journal of steroid biochemistry and molecular biology, 112(4-5), 213-219 (2008-11-13)
Cytochrome P450scc (CYP11A1) metabolizes vitamin D3 to 20-hydroxyvitamin D3 as the major product, with subsequent production of dihydroxy and trihydroxy derivatives. The aim of this study was to determine whether cytochrome P450scc could metabolize 1alpha-hydroxyvitamin D3 and whether products were