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Merck
CN

F9762

Sigma-Aldrich

抗-人IgM(μ-链特异性)−FITC 山羊抗

affinity isolated antibody, buffered aqueous solution

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MDL编号:
UNSPSC代码:
12352203
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生物来源

goat

偶联物

FITC conjugate

抗体形式

affinity isolated antibody

抗体产品类型

secondary antibodies

克隆

polyclonal

表单

buffered aqueous solution

储存条件

protect from light

技术

immunofluorescence: suitable (1:32 - 1:64 using Hep2 cells)

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Human IgM (μ-chain specific)-FITC antibody specifically binds to mu-chain of human IgM. After immunospecific purification, anti-human IgM is conjugated to fluorescein isothiocyanate (FITC), in an alkaline reaction. The antibody preparation is specific for human IgM when tested against purified human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins. The product is also determined to have no cross reactivity with mouse or rat serum proteins.

免疫原

Purified human IgM

应用

Anti-Human IgM (μ-chain specific)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:64 using human peripheral blood lymphocytes. A dilution of 1:32-1:64 may be used for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. A dilution of 1:32 may be used on acetone-fixed mouse liver cells for anti-nuclear antibody assay. It may also be used for agar diffusion assay.

生化/生理作用

No cross-reaction with mouse and rat IgG.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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分析证书(COA)

Lot/Batch Number

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E J Wiersma et al.
Journal of immunology (Baltimore, Md. : 1950), 160(12), 5979-5989 (1998-06-24)
Previous studies have discerned two forms of polymeric mouse IgM: moderately cytolytic (complement-activating) pentamer, which contains J chain, and highly cytolytic hexamer, which lacks J chain. To investigate the relationships among polymeric structure, J chain content, and cytolytic activity, we

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