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Merck
CN

G0400

L -谷氨酸氧化酶 来源于链霉菌

lyophilized powder, ≥5.0 units/mg solid

别名:

L -谷氨酸:氧氧化还原酶(脱氨)

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化学文摘社编号:
UNSPSC Code:
12352204
MDL number:
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form

lyophilized powder

specific activity

≥5.0 units/mg solid

composition

Protein, ≥15% Lowry

storage temp.

2-8°C

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Biochem/physiol Actions

Major enzyme in the synthesis or degradation of glutamic acid; transfers an amine to α-ketoglutaric acid to form L-glutamic acid or deamidates L-glutamic acid

Physical form

Lyophilized powder containing potassium phosphate buffer salts and lactose

Other Notes

One unit will form 1.0 μmole of α-ketoglutaric acid from L-glutamic acid per min at pH 7.4 at 30 °C.

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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分析证书(COA)

Lot/Batch Number

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Fumihiro Sassa et al.
Analytical chemistry, 80(16), 6206-6213 (2008-07-17)
A microfluidic device and operation to handle liquid plugs for biochemical analyses were developed for efficient handling of plugs of many solutions. A major part of the device was a T-junction consisting of a main flow channel and a handling
N E Katsos et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 807(2), 277-285 (2004-06-19)
Glutamate oxidase (GOX, EC 1.4.3.11) from Streptomyces catalyses the oxidation of L-glutamate to alpha-ketoglutarate. Its kinetic constants for L-glutamate were measured equal to 2 mM for Km and 85.8 s(-1) for kcat. BLAST search and amino acid sequence alignments revealed
Anjan Kumar Basu et al.
Biosensors & bioelectronics, 21(10), 1968-1972 (2005-11-18)
A monosodium glutamate (MSG) biosensor made by co-immobilized L-glutamate oxidase (L-GLOD) and L-glutamate dehydrogenase (L-GLDH) as the bio-component based on substrate recycling for highly sensitive MSG or L-glutamate determination, has been developed. Regeneration of MSG by substrate recycling provided an
Hong-Xuan Ren et al.
Talanta, 78(4-5), 1371-1377 (2009-04-14)
A novel matrix for enzyme immobilization was successfully developed by using Pt/Au bimetallic hierarchical structure with micro/nano-array to generate highly sensitive biosensors for glutamic oxaloacetic transaminase (GOT, EC 2.6.1.1) and glutamic pyruvic transaminase (GPT, EC 2.6.1.2) determination. The structures of
Faming Tian et al.
Analytica chimica acta, 645(1-2), 86-91 (2009-06-02)
We have developed an amperometric microbiosensor for real time monitoring L-glutamate release in neural tissue, based on enzymatic oxidation catalyzed by the L-glutamate oxidase. By means of a sol-gel coating method, L-glutamate oxidase was entrapped in a biocompatible gel layer

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